Primer reporte de hongos ophiostomatoides asociados con Ips calligraphus (Germar, 1823) (Coleoptera: Curculionidae) infestando Pinus occidentalis Sw. en la vertiente noroeste de la Cordillera Central, República Dominicana
Issue | Vol. 7 Núm. 2 (2024): Ciencia, Ambiente y Clima |
DOI | |
Publicado | feb 10, 2025 |
Estadísticas |
Universidad ISA. Av. Pres. Antonio Guzmán Fernández km 5 ½ La Herradura, Santiago de los Caballeros, República Dominicana
Universidad ISA. Av. Pres. Antonio Guzmán Fernández km 5 ½ La Herradura, Santiago de los Caballeros, República Dominicana.
Resumen
Ips calligraphus es la plaga principal de los bosques de Pinus occidentalis Sw. en República Dominicana y actúa como vector potencial de hongos ophiostomatoides. La asociación simbiótica de Ips con hongos ophiostomatoides puede alterar la defensa del árbol huésped al superar la toxicidad de los metabolitos secundarios, contribuyendo al éxito de colonización y reproducción del escarabajo y constituyéndose en un dúo letal para las coníferas y de grandes pérdidas económicas para la industria forestal. Por tanto, resulta crítico identificar las especies de hongos involucradas y comprender sus implicaciones para el manejo de los focos de infestación por I. caligraphus. En esta investigación, se aislaron hongos en medio de cultivo agar extracto de malta, a partir del cuerpo de insectos recolectados en árboles atacados. Los hongos fueron identificados con base en los atributos morfológicos del micelio y secuenciación de ADN usando cebadores ITS1-ITS4. Se identificaron seis morfotipos de hongos ophiostomatoides: cuatro Ophiostoma ips, un Leptographium manifestum y un Ophiostoma spp. Este es el primer reporte de hongos ophiostomatoides asociados a I. calligraphus en el país.
Barnett, H. L., & Hunter, B. (1998). Illustrated genera of imperfect fungi (4th ed.). Minnesota, US: Burgess Publishing Company, APS Press.
Billings, R.F. 1997 Ataque de gorgojo de la corteza de pino (IPS) en la República Dominicana Pp 131- 144 Seminario Nacional sobre Reforestación y Manejo de Bosques. Plan Sierra, 29 septiembre de 1997, San José de Las Mata, República Dominicana.
CABI. (2023). Ips calligraphus (six-spined engraver) Invasive Species Compendium. Retrieved from https://www.cabi.org/isc
Cervantes-Martínez, R., Cerano-Paredes, J., Sánchez-Martínez, G., Villanueva-Díaz, J., Esquivel-Arriaga, G., Cambrón-Sandoval, V. H., ... & Castruita-Esparza, L. U. (2019). Historical bark beetle outbreaks in Mexico, Guatemala and Honduras (1985–2015) and their relationship with droughts. Revista Chapingo Serie Ciencias Forestales y del Ambiente, 25(2), 269–290. https://doi.org/10.5154/r.rchscfa.2019.01.006
Christiansen, E., & Bakke, A. (1988). The spruce bark beetle of Eurasia. In A. A. Berryman (Ed.), Dynamics of forest insect populations: Patterns, causes, implications (pp. 479–503). Springer. https://doi.org/10.1007/978-1-4899-0789-9
Davydenko, K., Vasaitis, R., & Menkis, A. (2017). Fungi associated with Ips acuminatus (Coleoptera: Curculionidae) in Ukraine with a special emphasis on pathogenicity of ophiostomatoid species. European Journal of Entomology, 114 77–88. https://doi.org/10.14411/eje.2017.011
De Beer, Z. W., Wingfield, M. J., & Roux, J. (2014). Taxonomy and pathology of Ophiostoma and related fungi. Fungal Diversity, 70(1), 15–36. https://doi.org/10.1007/s13225-014-0294-y
Franceschi, V. R., Krokene, P., Christiansen, E., & Krekling, T. (2005). Anatomical and chemical defenses of conifer bark against bark beetles and other pests. New Phytologist, 167(2), 353–376. https://doi.org/10.1111/j.1469-8137.2005.01436.x
Haack, R. A., Wilkinson, R. C., Foltz, J. L., & Corneil, J. A. (1984). Gallery construction and oviposition by Ips calligraphus (Coleoptera: Scolytidae) in relation to slash pine phloem thickness and temperature. The Canadian Entomologist, 116(4), 625–632. https://doi.org/10.4039/Ent116625-4
Hammerbacher, A., Ralph, S. G., Bohlmann, J., Fenning, T. M., Gershenzon, J., & Schmidt, A. (2011). Biosynthesis of the major tetrahydroxystilbenes in spruce, astringin and isorhapontin, proceeds via resveratrol and is enhanced by fungal infection. Plant Physiology, 157(2), 876–890. https://doi.org/10.1104/pp.111.181420
Hammerbacher, A., Schmidt, A., Wadke, N., Wright, L. P., Schneider, B., Bohlmann, J., ... & Paetz, C. (2013). A common fungal associate of the spruce bark beetle metabolizes the stilbene defenses of Norway spruce. Plant Physiology, 162(3), 1324–1336. https://doi.org/10.1104/pp.113.218610
Hausner, G., Reid, J., & Klassen, G. R. (1993). On the phylogeny of Ophiostoma, Ceratocystis s.s., and Microascus and relationships within Ophiostoma based on partial ribosomal DNA sequences. Canadian Journal of Botany, 71(9), 1249–1265. https://doi.org/10.1139/b93-150
Jacobs, K., & Wingfield, M. J. (2001). Leptographium species: Tree pathogens, insect associates, and agents of blue-stain American Phytopathological Society (APS Press).
Jiménez, A. A., Cabrera, P. R. C., & Agramonte, J. E. (2022). Fluctuación poblacional de Ips calligraphus en pinares de la vertiente norte de la cordillera Central, República Dominicana. Novitates Caribaea, 20 137–154. https://doi.org/10.33800/nc.vi20.314
Jirošová, A., Modlinger, R., Hradecký, J., Ramakrishnan, R., Beránková, K., & Kandasamy, D. (2022). Ophiostomatoid fungi synergize attraction of the Eurasian spruce bark beetle, Ips typographus to its aggregation pheromone in field traps. Frontiers in Microbiology, 13 Article 980251. https://doi.org/10.3389/fmicb.2022.980251
Khadempour, L., LeMay, V., Jack, D., Bohlmann, J., & Breuil, C. (2012). The relative abundance of mountain pine beetle fungal associates through the beetle life cycle in pine trees. Microbial Ecology, 64 909–917. https://doi.org/10.1007/s00248-012-0077-z
Kirisits, T. (2004). Fungal associates of European bark beetles with special emphasis on the Ophiostomatoid fungi. In Bark and wood boring insects in living trees in Europe: A synthesis (pp. 181–236).
Kirisits, T. (2007). Fungal associates of European bark beetles with special emphasis on the Ophiostomatoid fungi. In Lieutier, F., Day, K. R., Battisti, A., Grégoire, J.-C., & Evans, H. F. (Eds.), Bark and wood boring insects in living trees in Europe: A synthesis (pp. 181–236). Springer. https://doi.org/10.1007/978-1-4020-2241-8_10
Kirisits, T., & Offenthaler, I. (2002). Xylem sap flow of Norway spruce after inoculation with the blue-stain fungus Ceratocystis polonica Plant Pathology, 51 359–364. https://doi.org/10.1046/j.1365-3059.2002.00722.x
Krokene, P. (2015). Conifer defense and resistance to bark beetles. In F. E. Vega & R. W. Hofstetter (Eds.), Bark beetles: Biology and ecology of native and invasive species (pp. 177–207). Academic Press.
Krokene, P., & Solheim, H. (1996). Fungal associates of five bark beetle species colonizing Norway spruce. Canadian Journal of Forest Research 26(12), 2115-2122. https://doi.org/10.1139/x26-240
Lanier, G. N., Teale, S. A., & Pajares, J. A. (1991). Biosystematics of the genus Ips (Coleoptera: Scolytidae) in North America: Review of the Ips calligraphus group. The Canadian Entomologist, 123(5), 1103–1124.
Levieux, J., Lieutier, F., Moser, J. C., & Perry, T. J. (1989). Transportation of phytopathogenic fungi by the bark beetle Ips sexdentatus Boerner and associated mites. Journal of Applied Entomology, 108(1–5), 1–11. https://doi.org/10.1111/j.1439-0418.1989.tb00425.x
Massoumi Alamouti, S., Kim, J. J., Humble, L. M., Uzunovic, A., & Breuil, C. (2007). Ophiostomatoid fungi associated with the northern spruce engraver, Ips perturbatus in western Canada. Antonie van Leeuwenhoek, 91 19–34. https://doi.org/10.1007/s10482-006-9092-8
Moreno-Rico, O., Sánchez-Martínez, G., Marmolejo-Monsiváis, J. G., Pérez-Hernández, K., & Moreno-Manzano, C. E. (2015). Diversidad de hongos ophiostomatoides en pinos de la Sierra Fría de Aguascalientes, México, asociados con Dendroctonus mexicanus Revista Mexicana de Biodiversidad, 86(1), 1–8. https://doi.org/10.7550/rmb.46751
Moser, J. C., Macías-Sámano, J. E., Konrad, H., & Kirisits, T. (1997). Phoretic mites and their hyperphoretic fungi associated with flying Ips typographus japonicus Niijima (Col., Scolytidae) in Japan. Journal of Applied Entomology, 133(9–10), 702–710. https://doi.org/10.1111/j.1439-0418.2009.01406.x
Pashenova, N. V., Seraya, L. G., & Baranchikov, Y. N. (2018). Transmission of the Ophiostoma fungi by Ips typographus in the Moscow region. Fungal Biology
Romón, P., Zhou, X., Iturrondobeitia, J. C., Wingfield, M. J., & Goldarazena, A. (2007). Ophiostoma species (Ascomycetes: Ophiostomatales) associated with bark beetles (Coleoptera: Scolytinae) colonizing Pinus radiata in northern Spain. Canadian Journal of Microbiology, 53(7), 756–767. https://doi.org/10.1139/W07-001
Runlei, C., Tuan, A. D., Stephen, J. T., Michael, J. W., Xudong, Z., & Mingliang, Y. (2018). Ophiostomatoid fungi associated with the spruce bark beetle Ips typographus including 11 new species from China. Persoonia https://doi.org/10.3767/persoonia.2019.42.03
Six, D. L., & Wingfield, M. J. (2011). The role of phytopathogenicity in bark beetle–fungus symbioses: A challenge to the classic paradigm. Annual Review of Entomology, 56(1), 255–272.
Upadhyay, H. P. (1981). A monograph of Ceratocystis and Ceratocystiopsis. University of Georgia Press.
Wermelinger, B. (2004). Ecology and management of the spruce bark beetle Ips typographus—A review of recent research. Forest Ecology and Management, 202(1–3), 67–82.
Wood, S. L., & Stark, R. W. (1968). The life history of Ips calligraphus (Coleoptera: Scolytidae) with notes on its biology in California the Canadian Entomologist, 100(2), 145–151. https://doi.org/10.4039/Ent100145-2
Zhao, L., Ahmad, F., Lu, M., Zhang, W., Wickham, J. D., & Sun, J. (2018). Ascarosides promote the prevalence of ophiostomatoid fungi and an invasive pathogenic nematode, Bursaphelenchus xylophilus Journal of Chemical Ecology, 44 701–710.
Zheng, W., Liu, Y., Wang, H. M., Meng, X., Liu, X. W., Decock, C., Zhang, X., & Lu, Q. (2020). Ophiostomatoid fungi associated with Ips subelongatus including eight new species from northeastern China. Mycological Progress https://doi.org/10.1186/s43008-019-0025-3
Billings, R.F. 1997 Ataque de gorgojo de la corteza de pino (IPS) en la República Dominicana Pp 131- 144 Seminario Nacional sobre Reforestación y Manejo de Bosques. Plan Sierra, 29 septiembre de 1997, San José de Las Mata, República Dominicana.
CABI. (2023). Ips calligraphus (six-spined engraver) Invasive Species Compendium. Retrieved from https://www.cabi.org/isc
Cervantes-Martínez, R., Cerano-Paredes, J., Sánchez-Martínez, G., Villanueva-Díaz, J., Esquivel-Arriaga, G., Cambrón-Sandoval, V. H., ... & Castruita-Esparza, L. U. (2019). Historical bark beetle outbreaks in Mexico, Guatemala and Honduras (1985–2015) and their relationship with droughts. Revista Chapingo Serie Ciencias Forestales y del Ambiente, 25(2), 269–290. https://doi.org/10.5154/r.rchscfa.2019.01.006
Christiansen, E., & Bakke, A. (1988). The spruce bark beetle of Eurasia. In A. A. Berryman (Ed.), Dynamics of forest insect populations: Patterns, causes, implications (pp. 479–503). Springer. https://doi.org/10.1007/978-1-4899-0789-9
Davydenko, K., Vasaitis, R., & Menkis, A. (2017). Fungi associated with Ips acuminatus (Coleoptera: Curculionidae) in Ukraine with a special emphasis on pathogenicity of ophiostomatoid species. European Journal of Entomology, 114 77–88. https://doi.org/10.14411/eje.2017.011
De Beer, Z. W., Wingfield, M. J., & Roux, J. (2014). Taxonomy and pathology of Ophiostoma and related fungi. Fungal Diversity, 70(1), 15–36. https://doi.org/10.1007/s13225-014-0294-y
Franceschi, V. R., Krokene, P., Christiansen, E., & Krekling, T. (2005). Anatomical and chemical defenses of conifer bark against bark beetles and other pests. New Phytologist, 167(2), 353–376. https://doi.org/10.1111/j.1469-8137.2005.01436.x
Haack, R. A., Wilkinson, R. C., Foltz, J. L., & Corneil, J. A. (1984). Gallery construction and oviposition by Ips calligraphus (Coleoptera: Scolytidae) in relation to slash pine phloem thickness and temperature. The Canadian Entomologist, 116(4), 625–632. https://doi.org/10.4039/Ent116625-4
Hammerbacher, A., Ralph, S. G., Bohlmann, J., Fenning, T. M., Gershenzon, J., & Schmidt, A. (2011). Biosynthesis of the major tetrahydroxystilbenes in spruce, astringin and isorhapontin, proceeds via resveratrol and is enhanced by fungal infection. Plant Physiology, 157(2), 876–890. https://doi.org/10.1104/pp.111.181420
Hammerbacher, A., Schmidt, A., Wadke, N., Wright, L. P., Schneider, B., Bohlmann, J., ... & Paetz, C. (2013). A common fungal associate of the spruce bark beetle metabolizes the stilbene defenses of Norway spruce. Plant Physiology, 162(3), 1324–1336. https://doi.org/10.1104/pp.113.218610
Hausner, G., Reid, J., & Klassen, G. R. (1993). On the phylogeny of Ophiostoma, Ceratocystis s.s., and Microascus and relationships within Ophiostoma based on partial ribosomal DNA sequences. Canadian Journal of Botany, 71(9), 1249–1265. https://doi.org/10.1139/b93-150
Jacobs, K., & Wingfield, M. J. (2001). Leptographium species: Tree pathogens, insect associates, and agents of blue-stain American Phytopathological Society (APS Press).
Jiménez, A. A., Cabrera, P. R. C., & Agramonte, J. E. (2022). Fluctuación poblacional de Ips calligraphus en pinares de la vertiente norte de la cordillera Central, República Dominicana. Novitates Caribaea, 20 137–154. https://doi.org/10.33800/nc.vi20.314
Jirošová, A., Modlinger, R., Hradecký, J., Ramakrishnan, R., Beránková, K., & Kandasamy, D. (2022). Ophiostomatoid fungi synergize attraction of the Eurasian spruce bark beetle, Ips typographus to its aggregation pheromone in field traps. Frontiers in Microbiology, 13 Article 980251. https://doi.org/10.3389/fmicb.2022.980251
Khadempour, L., LeMay, V., Jack, D., Bohlmann, J., & Breuil, C. (2012). The relative abundance of mountain pine beetle fungal associates through the beetle life cycle in pine trees. Microbial Ecology, 64 909–917. https://doi.org/10.1007/s00248-012-0077-z
Kirisits, T. (2004). Fungal associates of European bark beetles with special emphasis on the Ophiostomatoid fungi. In Bark and wood boring insects in living trees in Europe: A synthesis (pp. 181–236).
Kirisits, T. (2007). Fungal associates of European bark beetles with special emphasis on the Ophiostomatoid fungi. In Lieutier, F., Day, K. R., Battisti, A., Grégoire, J.-C., & Evans, H. F. (Eds.), Bark and wood boring insects in living trees in Europe: A synthesis (pp. 181–236). Springer. https://doi.org/10.1007/978-1-4020-2241-8_10
Kirisits, T., & Offenthaler, I. (2002). Xylem sap flow of Norway spruce after inoculation with the blue-stain fungus Ceratocystis polonica Plant Pathology, 51 359–364. https://doi.org/10.1046/j.1365-3059.2002.00722.x
Krokene, P. (2015). Conifer defense and resistance to bark beetles. In F. E. Vega & R. W. Hofstetter (Eds.), Bark beetles: Biology and ecology of native and invasive species (pp. 177–207). Academic Press.
Krokene, P., & Solheim, H. (1996). Fungal associates of five bark beetle species colonizing Norway spruce. Canadian Journal of Forest Research 26(12), 2115-2122. https://doi.org/10.1139/x26-240
Lanier, G. N., Teale, S. A., & Pajares, J. A. (1991). Biosystematics of the genus Ips (Coleoptera: Scolytidae) in North America: Review of the Ips calligraphus group. The Canadian Entomologist, 123(5), 1103–1124.
Levieux, J., Lieutier, F., Moser, J. C., & Perry, T. J. (1989). Transportation of phytopathogenic fungi by the bark beetle Ips sexdentatus Boerner and associated mites. Journal of Applied Entomology, 108(1–5), 1–11. https://doi.org/10.1111/j.1439-0418.1989.tb00425.x
Massoumi Alamouti, S., Kim, J. J., Humble, L. M., Uzunovic, A., & Breuil, C. (2007). Ophiostomatoid fungi associated with the northern spruce engraver, Ips perturbatus in western Canada. Antonie van Leeuwenhoek, 91 19–34. https://doi.org/10.1007/s10482-006-9092-8
Moreno-Rico, O., Sánchez-Martínez, G., Marmolejo-Monsiváis, J. G., Pérez-Hernández, K., & Moreno-Manzano, C. E. (2015). Diversidad de hongos ophiostomatoides en pinos de la Sierra Fría de Aguascalientes, México, asociados con Dendroctonus mexicanus Revista Mexicana de Biodiversidad, 86(1), 1–8. https://doi.org/10.7550/rmb.46751
Moser, J. C., Macías-Sámano, J. E., Konrad, H., & Kirisits, T. (1997). Phoretic mites and their hyperphoretic fungi associated with flying Ips typographus japonicus Niijima (Col., Scolytidae) in Japan. Journal of Applied Entomology, 133(9–10), 702–710. https://doi.org/10.1111/j.1439-0418.2009.01406.x
Pashenova, N. V., Seraya, L. G., & Baranchikov, Y. N. (2018). Transmission of the Ophiostoma fungi by Ips typographus in the Moscow region. Fungal Biology
Romón, P., Zhou, X., Iturrondobeitia, J. C., Wingfield, M. J., & Goldarazena, A. (2007). Ophiostoma species (Ascomycetes: Ophiostomatales) associated with bark beetles (Coleoptera: Scolytinae) colonizing Pinus radiata in northern Spain. Canadian Journal of Microbiology, 53(7), 756–767. https://doi.org/10.1139/W07-001
Runlei, C., Tuan, A. D., Stephen, J. T., Michael, J. W., Xudong, Z., & Mingliang, Y. (2018). Ophiostomatoid fungi associated with the spruce bark beetle Ips typographus including 11 new species from China. Persoonia https://doi.org/10.3767/persoonia.2019.42.03
Six, D. L., & Wingfield, M. J. (2011). The role of phytopathogenicity in bark beetle–fungus symbioses: A challenge to the classic paradigm. Annual Review of Entomology, 56(1), 255–272.
Upadhyay, H. P. (1981). A monograph of Ceratocystis and Ceratocystiopsis. University of Georgia Press.
Wermelinger, B. (2004). Ecology and management of the spruce bark beetle Ips typographus—A review of recent research. Forest Ecology and Management, 202(1–3), 67–82.
Wood, S. L., & Stark, R. W. (1968). The life history of Ips calligraphus (Coleoptera: Scolytidae) with notes on its biology in California the Canadian Entomologist, 100(2), 145–151. https://doi.org/10.4039/Ent100145-2
Zhao, L., Ahmad, F., Lu, M., Zhang, W., Wickham, J. D., & Sun, J. (2018). Ascarosides promote the prevalence of ophiostomatoid fungi and an invasive pathogenic nematode, Bursaphelenchus xylophilus Journal of Chemical Ecology, 44 701–710.
Zheng, W., Liu, Y., Wang, H. M., Meng, X., Liu, X. W., Decock, C., Zhang, X., & Lu, Q. (2020). Ophiostomatoid fungi associated with Ips subelongatus including eight new species from northeastern China. Mycological Progress https://doi.org/10.1186/s43008-019-0025-3
Ophiostoma ips
ITS-1
ITS-4
Leptographium
Extracto de Malta Agar (MEA)
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Afiliaciones
Esclaudys Pérez González
Universidad ISA. Av. Pres. Antonio Guzmán Fernández km 5 ½ La Herradura, Santiago de los Caballeros, República Dominicana
Elí Misael Bobadilla-Peñaló
Universidad ISA. Av. Pres. Antonio Guzmán Fernández km 5 ½ La Herradura, Santiago de los Caballeros, República Dominicana.
Cómo citar
Pérez González, E., & Bobadilla-Peñaló, E. M. (2025). Primer reporte de hongos ophiostomatoides asociados con Ips calligraphus (Germar, 1823) (Coleoptera: Curculionidae) infestando Pinus occidentalis Sw. en la vertiente noroeste de la Cordillera Central, República Dominicana. Ciencia, Ambiente Y Clima, 7(2), 31–48. https://doi.org/10.22206/cac.2024.v7i2.3336